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October 21, 2017. The online journal of the Indian Council for Historical Research brings. Donate1,25-Dihydroxyvitamin D₃ decreases the levels of metastasis-associated protein 1 in PC3 prostate cancer cells.

Metastasis-associated protein 1 (MTA1) promotes carcinoma cell migration, invasion and metastasis in prostate cancer cells. 1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) regulates gene expression in various cell types, and its osteoporosis-suppressive action is more pronounced in bone. Given these findings, we investigated whether 1,25(OH)2D3 attenuated MTA1 in prostate cancer cells. First, MTA1 protein levels were analyzed in prostate cancer cells using western blot analysis and we found that 1,25(OH)2D3 dose-dependently decreased the expression of MTA1. Next, the mRNA expression of MTA1 was suppressed in 1,25(OH)2D3-treated PC3 cells in a dose-dependent manner, and MTA1 mRNA expression was significantly decreased in a time-dependent manner. Additionally, 1,25(OH)2D3 attenuated MTA1 binding to the AP-1 element in the promoter region of the MTA1 gene. Finally, we revealed that MTA1 knockdown using small interfering RNA (siRNA) significantly decreased MTA1 protein levels and this MTA1 knockdown inhibited the migration and invasion of prostate cancer cells. Therefore, our study suggested that the effect of 1,25(OH)2D3 on MTA1 may be associated with AP-1 repression.EU carbon floor price

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The precise level of the carbon floor price is set in Directive 2009/147 0b46394aab

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