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The development of methods for culturing plant tissues from embryogenic callus of any plant species is a major step towards their genetic transformation. The use of plant cell culture systems for the regeneration of such plant tissues is highly desirable, because they are technically simple, and can be scaled up. In light of the high costs associated with the synthesis of proteins of interest in plant cells, the culture of somatic embryos is a promising means of producing these molecules. Maintaining the plant cells in culture can be done simply through the use of liquid media, with no need to use complicated equipment. At the same time, the secretion of plant-specific proteins, which are overproduced in plant cell cultures, can be used to identify the function of these proteins. A high level of expression in plant cell cultures of targeted genes is the major bottleneck in the use of these systems for the production of recombinant proteins. Thus, better plant cell culture systems that lead to the expression of such proteins at high level are desirable. In this chapter we describe the protocol to develop embryogenic cotton callus and to achieve somatic embryogenesis and plant regeneration through tissue culture, and the process of recovery of transgenic cotton plants.
Title - A great heroPrice - $$Category - Childrens EntertainmentAuthor - Robert NewtonDescription - A great hero.
David, a tamil movie, has Vikram, Lara Dutta, Tabu, Neil Nitin Mukesh, Jiiva as leading cast in the film. The songs from the David were composed by Anirudh Ravichander. The Lyrics of the Songs from the movie David was penned by Lyricists such as Mohanrajan, Remo Fernandes, Yugabharati, Joyshanti and V Elango. The movie was directed by Bejoy Nambiar. The tamil movie David was released in the year 2013.
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